Cat. No.: MSCR-K1 | Free Sample (100 ml)
(e.g., MSCs from adipose tissue, bone marrow, etc.)
(e.g., Cell therapy, drug development, regenerative medicine, etc.)
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Frequently Asked Questions (FAQs)

Which cell types is MSC StemPrime suitable for?

MSC StemPrime is a 100% chemically defined, serum-free medium developed for the isolation and expansion of human mesenchymal stem cells (MSCs). It supports the cultivation of MSCs from umbilical cord, bone marrow, adipose tissue, and other tissue sources, as well as immortalized MSC lines.

Its optimized formulation ensures consistent growth, high viability, and stable MSC characteristics across various applications.

MSC StemPrime is suitable for both isolation and expansion of human MSCs. During cultivation, it preserves all essential MSC surface markers, maintains multipotency, and helps prevent unintended differentiation.

  • Supports MSC isolation from primary tissues
  • Enables efficient expansion with high cell viability
  • Designed for research and clinical applications

Yes, MSC StemPrime is suitable for isolating human MSCs from primary tissues, particularly from the umbilical cord.

During isolation, we recommend optionally adding 2.0 – 2.5% human serum to enhance cell adherence and viability. No coating is required for this step.

The optimal culture conditions depend on the expansion strategy and duration:

  • Defined expansion:
    MSC StemPrime can be used without additional supplementation in its chemically defined form, with coating using fibronectin- or vitronectin-based reagents.
  • Serum-supplemented expansion:
    Adding 2.0 – 2.5% human serum may be required to support optimal cell performance. Coating remains optional.

For specific recommendations, contact our experts at techservice@capricorn-scientific.com.

No, MSC StemPrime is specifically optimized for mesenchymal stem cells and is not suitable for:

  • Induced pluripotent stem cells (iPSCs)
  • Hematopoietic stem cells (HSCs)
  • Embryonic stem cells (ESCs)

Looking for a medium for iPSCs or other stem cell types? Feel free to contact us at techservice@capricorn-scientific.com to explore suitable options!

The MSC StemPrime Kit consists of:

  • A chemically defined basal medium
  • A recombinant growth factor supplement

Once supplemented, the medium is ready to use and requires no further additives.

It is serum-free, xeno-free, and animal component-free and already contains L-glutamine and phenol red.

If you require any specific customization or a tailored formulation to meet your unique needs, please feel free to contact us. Our team will be happy to discuss the options and find the best solution for your application.

No, to ensure optimal performance and consistency, MSC StemPrime is only available as a two-component kit.

The medium and supplement are precisely formulated to work together, providing reliable conditions for MSC culture.

  • Medium:
    Shelf life of 1 year from the manufacturing date when stored at +2°C to +8°C, protected from light.
  • Supplement:
    Shelf life of 1 year from the manufacturing date when stored at ≤ -15°C, protected from light.
  • After supplementation:
    Stable for 4 weeks when stored at +2 °C to +8 °C, protected from light.

No, both versions undergo rigorous testing to ensure:

  • Consistent MSC isolation and expansion
  • High viability while maintaining multipotency
  • Reliable batch-to-batch reproducibility

MSC StemPrime GMP Grade is produced under strict regulatory guidelines with additional documentation, making it suitable for clinical and translational applications.

MSC StemPrime is compatible with standard plastic cultureware.

For optimal MSC attachment, we recommend using fibronectin-based coating or similar adhesion-enhancing reagents.

Coating is recommended for optimal MSC attachment (e.g., fibronectin-based coatings).

However, MSC StemPrime can also be used without coating if supplemented with 2.0 – 2.5% human serum. The choice depends on your specific culture setup and cell adherence requirements.

No, MSC StemPrime is fully chemically defined, serum-free, xeno-free, and animal component-free.

For isolation, adding 2.0 – 2.5% human serum can support cell adherence and viability. For further information, please refer to the product information or contact our experts at techservice@capricorn-scientific.com.

No, MSC StemPrime is antibiotic-free.

When proper aseptic techniques are followed, antibiotics are unnecessary and may actually interfere with MSC function. Antibiotics can accumulate inside cells, altering their behavior and gene expression, which may impact experimental reproducibility and clinical applications.

For best results, we recommend using antibiotic-free conditions and strict sterile handling.

We understand that specific research applications may require a tailored formulation.

If you need MSC StemPrime without phenol red or have other customization requests, our team is happy to assist you.

Get in touch with our experts at techservice@capricorn-scientific.com to explore personalized solutions that meet your exact needs!

MSC StemPrime provides a defined, consistent, and high-performance environment for MSC culture.

  • 100% chemically defined formulation for reproducible results
  • Serum-free & xeno-free for controlled culture conditions
  • Optimized for MSC isolation, expansion & scalability
  • Available in Research and GMP Grade for different applications

With unmatched purity, consistency, and performance, MSC StemPrime is the top choice for researchers and clinicians seeking reliable MSC culture solutions.

For further information please refer to the product information or contact our experts at techservice@capricorn-scientific.com.