HEKventure T, Chemically Defined Medium for Transient Viral Vector and Recombinant Protein Production, w/o L-Glutamine

Description

The HEKventure T product line was developed to maximize transient expression in HEK 293 suspension cultures. It was developed by florabio AS, our expert partner in culture media development and optimization.

HEKventure T is a chemically defined, animal component-free, and hydrolysate-free medium for transient transfection of HEK293 cell lines in suspension. It was developed to enhance recombinant protein expression and viral vector production (e.g., adeno-associated virus (AAV) and lentiviral (LV) vectors). The medium is particularly suited for polymer-based transfection, such as with polyethylenimine (PEI), and transient viral vector expression.

Combined with HEKventure Transfection Reagents (HEKVT-K1, HEKVT-K2), which can be used to maximize transfection efficiency, the product lines offer a streamlined approach to process optimization.

Key Highlights:

• High Viability:
  >90% viable cells over multiple passages with VCDs of up to 4 × 10⁶ cells/ml.
• Consistently High Yields:
  High protein yields, particularly for viral vector production.
• Optimized for PEI Transfection:
  optimized ionic strength, specifically formulated to avoid interactions with PEI and DNA.